Fig. 6

Differential expression analysis across stages of AD. ANOVA analysis with Tukey post hoc FDR was performed for pairwise comparison of mean log2(ratio) differences between the 3 stages of AD (i.e., Control, AsymAD and AD) of N = 390 total case samples and plotted as a volcano. Significance threshold for counting of peptides was p < 0.05 (dashed horizontal line). Differentially expressed peptides for (a) AsymAD (N = 127) versus control (N = 133), (b) AD (N = 130) versus control, and (c) AD versus AsymAD are labeled by their gene symbols. (d) Counts of peptides with significant difference in any of the 3 dichotomous comparisons are presented as a Venn diagram. Full statistics from the ANOVA and Tukey post-hoc analysis is presented in the ANOVA table in the Data folder deposited on Synapse25.