Table 2 Summary of heifer produced contigs assemblies. For details about pipeline used in this study, refer to Fig. 2. *BUSCO analysis was performed on polished contigs, **Inspector Quality Value is calculated on reference alignment andreads alignment.
From: A Bos taurus sequencing methods benchmark for assembly, haplotyping, and variant calling
ARS-UCD1.2 | ONT wtdbg2 | 10X supernova | HiFi hifiasm | CLR wtdbg2 | |
|---|---|---|---|---|---|
Pipeline used | a | b | a | a | |
Data type | CLR | ONT | 10X Chromium | CCS | CLR |
Quantity | 80X | 58X | 95X | 40X | 43X |
Assembler | Falcon | Wtdbg2 | Supernova | Hifiasm | Wtdbg2 |
Number of contigs | 3 077 | 7 226 | 26 306 | 1 444 | 2 857 |
Total size | 2 700 000 000 | 2 701 288 401 | 2 627 892 463 | 3 244 632 679 | 2 631 921 359 |
N50 contigs length | 12 000 000 | 23 641 545 | 488 571 | 84 059 894 | 16 542 341 |
BUSCO | 95.7%* | C:70.2% | C:94.7% | C:95.9% | C:90.0% |
Inspector QV ** | 22.29 | 27.09 | 47.25 | 25.79 |
