Table 3 Summary of heifer polished assemblies. Only ONT contig assembly was polished as CCS and 10X are low error rate reads. As CLR assembly is better than ONT assembly, we can expect at least similar result after polishing step. For details about pipeline used in this study, refer to Fig. 2.
From: A Bos taurus sequencing methods benchmark for assembly, haplotyping, and variant calling
ARS-UCD1.2 | ONT wtdbg2 polished | |
|---|---|---|
Pipeline used | a | |
Data type | CLR/Illumina | ONT/10X |
Quantity | 80X/83X | 58X/95X |
Polisher | Quiver + Arrow + Pilon | Racon + Pilon |
Number of contigs | 2 596 | 5 783 |
Total size | 2 715 825 655 | 2 700 580 867 |
N50 contigs length | 25 896 116 | 23 984 524 |
BUSCO | C:95.7% | C:95.2% |
