Fig. 3 | Scientific Data

Fig. 3

From: Medium-throughput image-based phenotypic siRNA screen to unveil the molecular basis of B cell polarization

Fig. 3

Image analysis pipeline and definition of polarity indexes. (a) Representative wide-field image of B cells stimulated with antigens immobilized at the surface of beads. Inserts show zooms on B cell-bead pairs stained for IgG (bead), γ-Tubulin (centrosome), and Lamp1 (lysosomes). Nuclei were counterstained with DAPI. (b) Schematics depicting the calculation of centrosome and lysosome polarity indexes.

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