Fig. 6
Validation of the automated image analysis. (a) Representative images of control non-electroporated cells in contact with non-stimulating beads (BCR-ligand−) or stimulating beads (BCR-ligand+) in the same imaging conditions as the screen. B cell-bead pairs were stained for IgG (bead), γ-Tubulin (centrosome), and Lamp1 (lysosomes). (b,c) Centrosome and Lysosome raw polarity indexes from individual cells. Boxes in box plots extend from the 25th to the 75th percentiles, with a line at the median, whiskers extend from the 10th to the 90th percentiles, and individual dots denote outlier values. Kolmogorov-Smirnov test was used to assess the normal distribution of the dataset. Mann-Whitney test was used to assess statistical significance.
