Fig. 7
Screen quality check. (a) Numbers of cell-bead pairs per well and replicate. Boxes in box plots extend from the 25th to the 75th percentiles, with a line at the median, whiskers extend from the 10th to the 90th percentiles, and individual dots denote outlier values. Kolmogorov-Smirnov test was used to assess the normal distribution of the dataset. Kruskal-Wallis with Dunn’s correction for multiple comparisons was used to assess statistical significance. (b) Cumulative frequency in percentage of the numbers of cell-bead pairs per well in each replicate. (c,d) Centrosome and lysosome raw polarity indexes of non-targeting siRNA-treated B cells (well B01, light blue) incubated with stimulating beads per plate and replicate and compared to the extent of polarization observed in non-electroporated control B cells incubated with either stimulating (light red) or non-stimulating (grey) beads. Boxes in box plots extend from the 25th to the 75th percentiles, with a line at the median, whiskers extend from the 10th to the 90th percentiles, and individual dots denote outlier values. Kolmogorov-Smirnov test was used to assess the normal distribution of the dataset. Kruskal-Wallis with Dunn’s correction for multiple comparisons was used to assess statistical significance. (e,f) Correlation analysis of centrosome and lysosome median polarity indexes in replicate #1 versus replicate #2.
