Fig. 8
Validation of the screening strategy. (a,b) Centrosome and lysosome normalized polarity indexes extracted from the screening dataset for the selected hits previously described in the literature as regulators of cell polarity (Dynein and ESCRT-III subunits, respectively). (c) Primary B cells were purified from the spleens of Chmp2b+/+ and Chmp2b−/− mice and stimulated with BCR-ligand+ beads. Expression of Chmp2b was verified by Western blot. (d) Centrosome and lysosome polarity indexes from Chmp2b+/+ and Chmp2b−/− primary B cells stimulated with BCR-ligand+ beads. Boxes in box plots extend from the 25th to the 75th percentiles, with a line at the median, whiskers extend from the 10th to the 90th percentiles, and individual dots denote outlier values. Kolmogorov-Smirnov test was used to assess the normal distribution of the dataset. Mann-Whitney test was used to assess statistical significance.
