Fig. 2
From: A multi-omics dataset of human transcriptome and proteome stable reference
Results of mycoplasma detection in 5 cell lines. The marker used in (a,b) was DL500 DNA Marker (Takara, 3590Q, Japan), and the marker used in (c,d) was Low Ladder (Dongsheng Biotech, M1031, China). The concentration of agarose gel was 2% and the electrophoresis buffer was 1 × TBE. The electrophoresis conditions were: voltage: 100 V, current: 230 mA, electrophoresis time: 25~30 min. According to its user manual, the results of the cell samples were judged as follows: The DNA amplification product of the host cell was 150 bp band. When there were two bands of 280 bp and 150 bp, it represented mycoplasma positive. When there was only one 280 bp band, it represented mycoplasma contamination seriously. When there was only one 150 bp band, it was mycoplasma negative. When there was no band, the PCR reaction was inhibited.
