Fig. 6
From: A multi-omics dataset of human transcriptome and proteome stable reference
RNA-seq of degraded MHCC97H RNA samples. (a) The electrophoresis of the total RNA of MHCC97H which was frozen and thawed for 10 cycles and exposed to air for a prolonged time. (b) Correlation of the transcriptome quantification of the non-degraded and its corresponding degraded RNA samples by oligo-dT method. (c) Similar to Fig. 6b, but used the rRNA depletion method. (d) Artificially degraded MHCC97H total RNA by adding RNase A for 30 seconds to 1 hour. (e) Similar to Fig. 6c, for the RNaseA-degraded RNA. The marker used in Fig. 6 was DL2000 DNA Marker (Takara, 3427 A, Japan). The concentration of agarose gel was 1% and the electrophoresis buffer was 1 × TBE. The electrophoresis conditions were: voltage: 100 V, current: 230 mA, electrophoresis time: 25~30 min. (Note: 1~9 represent the generation 1~9 of the cell line, respectively).
