Fig. 3

(a–c) Depiction of N₅₀ values (blue colour) and percentage alignment (pink colour) of sequencing reads and reference genomes vis-à-vis Mash values discerned through the de-novo reference-based guided approach for E. coli strains (a) E. coli IP9 (b) E. coli KKA (c) E. coli IPE; (d) composite depiction of comparative N₅₀ values of the three E. coli strains using de-novo reference-based guided assembly (magenta dots) and de-novo assembly (green dots). The orange dot represents the N₅₀ value of the assembly when 75% of the sequence reads were aligned with reference genomes.