Fig. 8 | Scientific Data

Fig. 8

From: Integrated Transcriptome Analysis of Long Noncoding RNA and mRNA in Developing and Aging Mouse Retina

Fig. 8

Laboratory validation of lncRNA-otx2os1 with potential of regulating retinal differentiation. (a) RNA sequencing and (b) qRT-PCR (ANOVA, n = 6, ****P < 0.001) showed similar expession patterns of lncRNA-otx2os1 in retina in six periods. (c) Results of qRT-PCR showed lncRNA-otx2os1 highly expressed in retina compared to other five tissues. (d) Fluorescence in situ hybridization showed that fluorescence intensity of lncRNA-otx2os1 corresponded with quantification in RNA sequencing. LncRNA-otx2os1 mainly located in neuroblast layer or outer nuclear layer, and increased in inner nuclear layer and retinal ganglion cell layer after differentiation and development. CE: close eye period; SP: suckling period; PP: puberty period; AP: adult period; MP: middle period; OP: old period; d: days; w- weeks; GCL: ganglion cell layer; NBL: neuroblast layer; INL: inner nuclear layer; ONL: outer nuclear layer.

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