Fig. 1
From: Fully resolved assembly of Fusarium proliferatum DSM106835 genome
Flow diagram of the isolation, genome sequencing and assembly of Fusarium proliferatum DSM106835. Date palm trees showing symptoms of SDS were used to establish a pure culture of F. proliferatum DSM106835. Spores produced by the fungal pathogen were observed under light microscopy and further used for HMW DNA extraction. Omni-C and HiFi SMRbell libraries were prepared for Illumina HiSeq-X (short-read sequencing) and PacBio® Sequel II (long-read sequencing), respectively. HiFi and Omni-C reads were merged to develop a long-read-only assembly where all chromosomes were present as single contigs without the introduction of artificial gaps (Courtesy of Illumina, Inc., Pacific Biosciences of California, Inc.). SDS, sudden death syndrome; HMW, high molecular weight.
