Table 3 Sequencing assay and method information, coverage, and brain regions of collection 2967.

From: Genomic data resources of the Brain Somatic Mosaicism Network for neuropsychiatric diseases

Assay

Depth

Region

Num.

Kits

Sequencer

Enrichment

WGS

30x

CB

46

Illumina TruSeq PCR Free kit

HiSeq X

Bulk tissue

Dura

62

90x

DLPFC, HIPPO

40

Illumina TruSeq Nano kit

NovaSeq

Bulk tissue

90x

DLPFC

87

Illumina TruSeq Nano kit

HiSeq X

Prime Flow RNA Assay based enrichment (Excitatory, Inhibitory, Oligos, and Astrocyte/Microglia/Epithelial)

RNA-seq

80–100 M reads

DLPFC, HIPPO

89

Illumina TruSeq Stranded Total RNA Library Prep Gold

HiSeq. 3000

Bulk tissue

  1. The “Num.” column describes number of samples. In total, 108 unique subjects underwent sequencing. Samples were obtained from the DLPFC (dorsolateral prefrontal cortex), HIPPO (hippocampus), CB (cerebellum), and Dura (dura mater), and underwent WGS or RNA-seq.
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