Table 2 Mass spectrometer settings for peptide measurement corresponding to two sets of LC conditions.
From: Venom-gland transcriptomics and venom proteomics of the Tibellus oblongus spider
100 Å pore column/Short gradient | 300 Å pore column/Long gradient | |
|---|---|---|
Automatic gain control (MS) | 5 × 105 | 1 × 106 |
Dynamic exclusion time, s | 10 | 30 |
Top N (number of sequential MS/MS scans) | 10 | 7 |
Maximum injection time (MS/MS), ms | 200 | 250 |
Automatic gain control (MS/MS) | 5 × 104 | 2 × 105 |
Normalized collision energy | 28 | 30 |
