Fig. 1 | Scientific Data

Fig. 1

From: Unveiling Hypothalamic Molecular Signatures via Retrograde Viral Tracing and Single-Cell Transcriptomics

Fig. 1

Generation of a nuConnect-seq-derived dataset. (a) A retrograde nuConnect-seq approach was used to generate a single nuclei RNA-seq (snRNA-seq) dataset from CRH-IRES-Cre (CRH-Cre) mice. (b) PRVB180, a pseudorabies virus expressing TK (thymidine kinase)-GFP (green fluorescence protein) in the Cre+ neurons and their upstream. (c) The flow of nuConnect-seq method. (d) UMAP plot showing the manually curated cell types of the presented dataset. (e) Violin plot showing the relative expression of neuronal and non-neuronal gene markers.

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