Fig. 4
From: A Drosophila heart optical coherence microscopy dataset for automatic video segmentation

Comparison of the ground truth mask (red) and the FlyNet 2.0+ mask (blue). Each of the five panels shows a preview of Supplementary File 1–5. Within each panel, the top left quadrant shows the ground truth overlayed on the resized image, while the bottom right shows the two masks overlayed on each other to highlight differences in the segmentation. On the right is the IOU, the area of heart, and the heart rate for each frame plotted over time. (a–c) show a larva, an early pupa and an adult fly respectively with no light stimulation. (d,e) show optogenetics pacing experiments on an early pupa with an excitatory opsin, ReaChR and an inhibitory opsin, NpHR, respectively. The vertical red lines correspond to when the light pulses were executed. The heart rate increased or decreased to the designed frequency respectively.