Fig. 4
From: The extrachromosomal circular DNA atlas of aged and young mouse brains

Validation of the purification of mouse brain eccDNA. (a) The results of agarose gel electrophoresis demonstrated that no bands indicative of linear DNA was detected in the samples obtained from mouse brain tissue, except for the positive control (P1 and P2). (b) The results of agarose gel electrophoresis consistently exhibited bands corresponding to the amplified products of eccDNA after rolling circle amplification.