Fig. 1

Sampling and sequencing for the peanut aerial and subterranean pods. (a) The six developmental stages (S1–S6) of peanut aerial (Air) and subterranean (Und) pods. Air1 (S1) refers to the peg rather than a pod, and is a shared stage in both aerial and subterranean development. The S2 and S3 stages include a blend of pod shell, kernel, and seed coat samples, Air2 and Air3 for aerial pod, while Und2 and Und3 for subterranean pod. From the S4 stage onward, these tissues were sampled separately. Air4_1 and Air4_2 correspond to the aerial pod shell, and a combination of the kernel and seed coat, respectively; while Und4_1 and Und4_2 to the subterranean pod shell, and a combination of the kernel and seed coat, respectively. S5 stage referred to the immature pod, Air5_1, Air5_2 and Air5_3 correspond to the aerial pod shell, seed kernel and seed coat, respectively; while Und5_1, Und5_2 and Und5_3 correspond to the aerial pod shell, seed kernel and seed coat, respectively. S6 stage referred to the mature pod, Air6_1, Air6_2 and Air6_3 correspond to the aerial pod shell, seed kernel and seed coat, respectively; while Und6_1, Und6_2 and Und6_3 correspond to the aerial pod shell, seed kernel and seed coat, respectively. (b) Simplified pipeline of the RNA-seq process.