Fig. 1
Comparison of different characteristics between ncMAPs and cMAPs. (A) The workflow for identification of cMAPs and ncMAPs. (B) Difference of main characteristics between ncMAPs and cMAPs which was calculated as the median of cMAPs minus the median of ncMAPs. Red points indicate difference in favor of ncMAPs, blue points indicate in favor of cMAPs, and grey indicates no difference. (C,D) Boxplot showing the difference of PEP (posterior error probability) and T-cell contact hydrophobicity between ncMAPs and cMAPs. The PEP is a statistical measure that estimates the probability of a peptide being wrongly identified in proteomics analysis with MaxQuant. The Wilcoxon test was utilized for statistical analysis. (E) Pearson correlations between observed retention times and predicted retention time. (F) The length distribution of source ORFs. (G) The percentage distribution of the number of MAPs identified across different numbers of samples. The x-axis represents how many samples MAPs will be detected in, while the y-axis displays the percentage of MAPs appearing in n samples (n = 1, 2,…, 18).
