Fig. 6

The biomarker panel allows for flexible utility in multiple tissue section types, including needle biopsies (top row), and TMAs (bottom two rows). Tumour foci are clearly highlighted, and the variations in intensity in Sortilin and Syndecan-1 can assist with grading. The needle biopsy sample was initially graded as ISUP 1 using H&E and was later confirmed to be ISUP 1 using the biomarker panel. Within each core, Appl1 labelling identified both benign and suspicious glands. These glands when assessed with Sortilin and Syndecan-1 and confirmed to have well-formed morphology consistent with ISUP 1. The TMA was originally graded as ISUP 3 by H&E. The first core contains both well-formed and poorly-formed malignant glands, however the proportion of poorly-formed morphology is larger than well-formed. Similarly, poorly-formed glands specifically cribriform glands predominated with smaller regions of well-formed malignant glands correlating with the Sortilin and Syndecan-1 labelling of the cores and an ISUP 3 grade group. No benign glands were evident in either the H&E stained or IHC labelled TMA cores.