Fig. 2
From: High-throughput drug screening identifies novel therapeutics for Low Grade Serous Ovarian Carcinoma
Schematic depicting the workflows for establishing cell line growth kinetics and high-throughput drug screening. Firstly, cell line growth kinetics and optimal seeding density were established through live cell imaging (IncuCyte). For high-throughput drug screening experiments, cell lines were seeded in 384 well format and left to adhere for a minimum of 20 hr. The following day test compounds were rehydrated in cell culture media and control drugs prepared, with all compounds stamped onto cell plates using automated liquid handling platforms. Following 72 hr of incubation cell plates were stained with DAPI to determine viability, and imaged for data processing.
