Fig. 7
Impact of sample‐wise and library‐wise abundance filtering thresholds on the mean richness (absolute and relative), abundance (absolute and relative), and prevalence of fungal operational taxonomic units (OTUs): (a) Phylum Ascomycota, (b) phylum Basidiomycota, (c) subkingdom Mucoromyceta (comprising phyla Calcarisporiellomycota, Glomeromycota, Mortierellomycota and Mucoromycota), and (d) early‐diverging lineages of zoosporic fungi (predominantly phyla Rozellomycota and Chytridiomycota). Sample‐wise thresholds are shown along the x‐axis, and paired boxes represent library‐wise thresholds of 0.5% (on the left) and 1.0% (on the right). Panels (e,f) demonstrate the impact of sample‐wise filtering on global relative richness and abundance, based on the 0.5% library‐wise abundance filter, which produced a marked effect on richness and a marginal effect on relative sequence abundance at high taxonomic resolution.
