Fig. 2

Schematic diagram of the study. A schematic representation of the study is presented. For A. mellifera, worker samples from larva to adult and queen samples from larvae to pupae were prepared. Total RNA was extracted from the samples and used for RNA-Seq. Using RNA-Seq data, expression data for all previously prepared transcripts in the prepared samples were constructed. For A. cerana japonica, larva to adult worker samples were prepared. Using the RNA-seq data, transcript sequence data, predicted amino acid sequence data, and functional annotation data were constructed. Furthermore, the expression data of the transcripts in the prepared samples were constructed using RNA-Seq and transcript sequence data.