Table 2 Primer sequences for Giardia duodenalis genome coverage detection.

Primer sequence	Primers (5′–3′)	Size (bp)	Annealing temperature (°C)
1	F: TCAGACACAGCAGATAGTAAG	821	50
1	R: TCACCTCAAGCAGCAGTA	821	50
2	F: ACGAAGACGAAGCGATAC	752	50
2	R: CTACAACGAAGACGAACCT	752	50
3	F: TCCATTCGTGACCATATCG	520	50
3	R: ATACTGATGTGACCGTTAGG	520	50
4	F: AGCCTCCTCCAACACTATA	957	50
4	R: TGACAGCGTATGCGATAG	957	50
5	F: CATTGTGTAGGTAGGCAGAA	991	50
5	R: TTAGAGGCGTGGATGACT	991	50
6	F: GCATTATCTGGCGGTATCA	799	50
6	R: GAGGAGCATAGGATTCACAA	799	50
7	F: GGACCTCCTCTCAACAAC	508	50
7	R: AGTGTAATGCCAGAGAATGA	508	50
8	F: GCTCCTGACTGAAGATTGT	596	50
8	R: GCGATTGATGACGATATACC	596	50
9	F: CTCAAGCATCGGCAGTAA	609	50
9	R: AAGCAGTCTACAGCAAGTAA	609	50
10	F: GAAGAAGGTGAGCGTGTT	624	50
10	R: CTGCCGATTGATGGATGT	624	50
11	F: GATAGCATCCGCACGAAT	968	50
11	R: TACTTCCTGTCTCTTCCTCT	968	50
12	F: TGATACAGGTGGCAAGGT	558	50
12	R: TAGGAAGTGAAGAGGAAGGA	558	50
13	F: CATCACATCCAGAGTAGAAGA	860	50
13	R: CACATAAGCACGCAGAGA	860	50
14	F: TGAGATAGTTGATGGCAGAC	682	50
14	R: TGACCGATTGGATGGAGTA	682	50
15	F: TAACGGTGGATGACTATGAC	562	50
15	R: CGACGGAAGTGGATTGAA	562	50
bg	F1: AAGCCCGACGACCTCACCCGCAGTGC	511	65
	R1: GAGGCCGCCCTGGATCTTCGAGACGAC		65
	F2: BG3 GAACGAACGAGATCGAGGTCCG		55
	R2: BG4 CTCGACGAGCTTCGTGTT		55
*gdh*	F1: TTCCGTRTYCAGTACAACTC	520	50
	R1: ACCTCGTTCTGRGTGGCGCA		50
	F2: ATGACYGAGCTYCAGAGGCACGT		50
	R2: GTGGCGCARGGCATGATGCA		50
*tpi*	F1: AAATIATGCCTGCTCGTCG	530	50
	R1: CAAACCTTITCCGCAAACC		50
	F2: CCCTTCATCGGIGGTAACTT		50
	R2: GTGGCCACCACICCCGTGCC		50

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