Table 1 Batch genomic DNA (gDNA) isolation for distribution.

From: Development and extensive sequencing of a broadly-consented Genome in a Bottle matched tumor-normal pair

Sample Input

Measurement Method

HG008-T

HG008-N-D

HG008-N-P

5 million non-viable tumor cells from batch 0823p23

two separate isolations of 10 mg of normal duodenal tissue were performed

two separate isolations of 10 mg of normal pancreatic tissue were performed

Genomic DNA Yield

Qubit

60 µg

34 µg (18 µg and 16 µg)

16 µg (5 µg and 11 µg)

Read N50

ONT sequencing

30 kb

10 kb

8 kb

Sizing

FemtoPulse

58 kb

NA

26 kb

Tapestation

NA

53 kb

NA

  1. DNA isolations from the three HG008 samples were performed for distribution to certain laboratories for measurement. Isolated DNA was sent to several sites for genome-scale measurements as described in Table 2. To confirm gDNA was fit for purpose, yield and length of gDNA was measured at different sites prior to use. Sizing and N50 reported are approximated values from three measurement sites. Genomic DNA yield from normal tissues for the separate isolations is noted in parentheses.
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