Fig. 3
Behavioral performance in task training sessions. (a,b) Schematic illustrations of the lever-pull task for head-fixed mice. In response to the sound cue, mice should pull the lever and hold it for a specified duration to receive a water reward from the lick spout (a). Premature pulls before the sound cue caused an additional delay in sound cue presentation. If the lever was pulled within 1 s after the sound cue, and the position exceeded 1 mm (threshold) for the predetermined duration (Tpull; see Methods for details), the lever pull was considered successful, and a water reward was provided. An insufficient pull or no response within 1 s after the sound cue was considered a failure. (c) Response rate (number of valid pulls/number of sound cues; top panel), success rate (number of successful pulls/number of sound cues; middle panel), and final pull duration (Tpull_final; see Methods for details; bottom panel) across sessions. Thin lines show performances of individual female (red) and male (gray) animals, whereas thick lines represent medians across the population (red, female; gray, male; black, all). (d) Color-coded response rates and Tpull_final across sessions. In some sessions, data were missing for various reasons (red crosses; 11/375 = 0.29%). The result of hierarchical clustering (Ward’s method) based on the chronological change in Tpull_final is shown alongside. (e) Behavioral metrics of individuals in cluster A (n = 7; magenta) and B (n = 18; cyan) identified in (d). These plots are shown similarly as in (c).
