Fig. 5
Validation of alignment to the Allen CCF reference atlas. (a,b) Validation of MesoNet-based skull-landmark inference. (a) Aligned skull landmarks from Allen CCF (orange) are plotted on the animal-average image of a representative animal and are compared with the landmarks being maƒnually annotated on three representative sessions from the animal (cyan). (b) Manually annotated skull landmarks are warped and plotted on the Allen CCF atlas (cyan, 75 sessions from 25 animals) and compared with the reference landmarks (black). (c) Coordinates and names of segmented brain regions used for the dataset, derived from the dorsal view of the Allen Common Coordinate Framework (version 3). Only the left hemisphere is shown; however, our dataset contained the ROIs of the right hemisphere as well. (d) Schematic illustration of the sensory-mapping experiments. Visual, auditory, and tactile stimulations were applied in the same task box as the task training sessions (see Methods for details). (e) Multi-modality sensory maps obtained from a representative mouse are shown (top panels). Color codes show normalized trial-averaged cortical responses (2 s duration from the stimulus onset) evoked by the three different sensory stimuli. Animal-averaged sensory maps of each modality (middle panels; n = 25 mice). Contours defined by more than 80th percentiles in the normalized map are displayed (bottom panels). In each animal and each sensory stimulus, the evoked responses were normalized in the range from 0 to 1. Contours from 25 animals are overlaid. For this analysis, the data from the first session of the sensory-mapping experiment in each animal are used.
