Fig. 7
Validation of task-related behavior and activity in a representative session. (a) Raw trajectories of calcium dynamics from various cortical regions and video-tracked body-part movements with sensor-based behavioral records (lever movement, gross body motion, and lick rate), referenced to task events (magenta, sound cue onset; blue, reward delivery). The disconnected part in the trace of the left forepaw is caused by the value deficit with the deviation from the 5th and 95th percentiles of DeepLabCut (DLC) estimation. (b) Lever-pull-aligned traces of calcium dynamics (left column) as well as video-tracked body-part movements and sensor-based behavioral records (right column). Trial-averaged (magenta) and single-trial (black) traces from a representative session are shown. The bottom-most traces of both columns show the same lever traces as references. (c) Trial-averaged calcium activity was mapped onto the Allen CCF and shown in chronological order from the top left to the bottom right. Calcium activity was binned into intervals of 10 frames (333 ms), and each bin was averaged. The labels (−1 s, 1 s, 2 s, 3 s) show time relative to lever-pull onset. The data are from the same session shown in (a,b).
