Fig. 2
From: Single-cell RNA sequencing dataset of hearts from wild type and Cyp26b1 knockout mouse embryos
Quality of the scRNA-seq dataset. (a) Schematic diagram of constructing Cyp26b1 global knockout mouse line with CRISPR/Cas9 technology. (b) Genotype validation of mice by PCR. WT: Wild type mice; KO: Knock out mice. (c) Representative H&E staining images of KO and WT cardiac coronal maximum section at E14.5. LA: Left atria; RA: Right atria; LA: Left ventricle; RA: Right ventricle; IVS: Interventricular septum. (d) Bar plot showing the cell numbers per sample. (e,f) Violin plots showing the number of unique molecular identifiers (UMIs) detected per cell (e) and the percentage of counts from mitochondrial genes (f), split by sample. (g) Bar plots showing the distribution of the number of unique genes per cell, across various samples. (h) Bar plot showing the estimated cell cycle phases across different time points. (i) Heatmap clustering of correlation coefficients across different samples. (j) UMAP plot showing the cell cluster distribution across the different samples.
