Abstract
Monochamus sutor (Coleoptera: Cerambycidae) is a pest that has led to significant economic and ecological losses. Herein, we created a high-quality chromosome-level reference genome for M. sutor by integrating MGI, Nanopore, and Hi-C sequencing technologies. We successfully assembled the scaffolds into eleven chromosomes with a total size of 762.16 MB, featuring a scaffold N50 of 84.3 Mb. In total, 381.54 Mb (50.06%) of repetitive elements were identified, and 14,404 protein-coding genes were predicted, with 83.08% of them annotated. The genome of M. sutor is an invaluable resource that provides data support for biological, ecological, genetic, and other research. It also offers a new basis to explore the functions of key gene families. More importantly, this genome enables data-driven risk prediction of pine wilt disease outbreaks, helping to address the vector role of M. sutor and support scientific surveillance strategies to reduce forest losses.
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Data availability
All data used in this study were generated through our own assembly. All relevant datasets have been uploaded to NCBI and Figshare and these datasets are publicly available. MGI second-generation sequencing data, Hi-C sequencing data, Nanopore sequencing raw data, and RNA-seq data were deposited in the Sequence Read Archive at NCBI (https://www.ncbi.nlm.nih.gov/sra/SRP597287). The final chromosome assembly, genome structure annotation, amino acid sequences and functional annotation results of protein-coding genes were deposited to Figshare repository under a DOI number of https://doi.org/10.6084/m9.figshare.c.7921310. The final chromosome assembly was deposited in GenBank (https://identifiers.org/insdc.gca:GCA_052757275.1).
Code availability
There were no custom scripts or code utilized in this study.
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Acknowledgements
This research was supported by the Science and Technology Innovation Program of Xiongan New Area (2023XAGG0065), Fundamental Research Funds for the Central Universities (QNTD202510).
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J.T. designed the study. D.Y., J.R.R. and C.Q.Z. contribute to the samples; D.Y. and M.L. established a platform for genome assembly. D.Y. contribute to the genome assembly and annotation. D.Y. wrote the draft manuscript. J.T. contributed to the revisions. The final manuscript has been read and approved by all authors.
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Yu, D., Li, M., Ren, JR. et al. Chromosome-level genome assembly of Monochamus sutor in China. Sci Data (2025). https://doi.org/10.1038/s41597-025-06348-z
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DOI: https://doi.org/10.1038/s41597-025-06348-z
