Fig. 1

Experimental design and multi-omics data generation. hESCs were differentiated into the endoderm germ layer and subsequently into polyhormonal cells. Samples were collected at 10 different time points during the time-course of hESC differentiation, and matched samples were measured for RNA, translation, and protein levels by RNA-seq, ribosome profiling, and mass spectrometry, respectively. Created with BioRender.com.