Table 6 Cell line information and culture conditions.

From: Multidimensional transcriptome dataset for systematic evaluation of Jakyakgamcho-tang-induced cell signatures

Cell

Cell line information

Medium for culture

Cell density (cells/well)

Description

Tissue

ATCC No.

IC20

RNA seq.

HepG2

Human hepatocellular carcinoma cell line

Liver

HB-8065

DMEM + 10% heat-inactivated FBS + antibiotics

2 × 104

6 × 105

PC12

Rat pheochromocytoma cell line

Adrenal gland

CRL-1721

DMEM + 10% heat-inactivated HS + 5% non-heat inactivated FBS + antibiotics

1 × 105

6 × 105

C2C12

Mouse myoblast cell line

Muscle

CRL-1772

DMEM + 10% heat-inactivated FBS + antibiotics

1 × 105

5 × 105

  1. For IC20 assay, 96-well (HepG2, 100 μL/well) or 24-well (PC12 and C2C12, 500 μL/well) culture plates were used. For RNA-seq sample preparation, 6-well (3 mL/well) culture plates were used for all cell lines.
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