Figure 4
From: Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness
IFT20 is required for assembly of the Golgi apparatus. (a) SaOS2 cells transfected with the indicated siRNAs were stained with antibodies against GM130 and γ-tubulin to visualize the cis-Golgi and centrosome (centriole pair), respectively, and counterstained with DAPI (blue). Insets show magnified images of boxed regions. Scale bar, 10 µm. (b) Number of Golgi fragments per cell was quantified and presented as a box-and-whisker plot. n = 69–74, three independent experiments; ***P < 0.001, t test. (c) Golgi dispersion in Ror2- or IFT20-knockdown cells can be suppressed by ectopic expression of IFT20. SaOS2 cells transfected with si-Ctrl, si-Ror2 or si-IFT20#1 were further transfected with pIRES2-ZsGreen1-sr-IFT20 (+) or pIRES2-ZsGreen1 (−), as indicated. Number of Golgi fragments in ZsGreen1-positive cells was quantified and presented as a box-and-whisker plot. n = 52–70, three independent experiments; *P < 0.05, ***P < 0.001, t test. (d) Effects of suppressed expression of Ror2 or IFT20 in SaOS2 cells on distribution of the cis-Golgi and TGN marker proteins. SaOS2 cells transfected with the indicated siRNAs were stained with antibodies against GM130 and Golgin-97 to visualize the cis-Golgi and TGN, respectively, and counterstained with DAPI (blue). Insets show magnified images of boxed regions. Scale bar, 10 µm.
