Figure 3
Long-lifespan related lipids. (a) Concentration level change and double bond distribution of long MLS predictors belonging to two lipid sub-classes in six tissues. Change in the concentration level is calculated as the difference between mean of the concentration levels in the long-living species and all other species in each of the clades. Left panel shows median concentration change of long MLS predictors belonging to simple Glc series and triacylglycerol lipid sub-classes in long-living species. The error bars show the 0.25 to 0.75 inter-quintile intervals. Significant concentration shift towards lower and higher values in long-living species is indicated by blue and red colors, respectively. Significant enrichment of MLS predictors among detected compounds in a given lipid sub-class is shown by thick lines and asterisks. The vertical dashed line shows the median concentration change for all long MLS predictors. Right panel shows the median double bond number and its 0.25 to 0.75 inter-quintile intervals for the same lipid sub-classes as in the left panel. Significantly higher or lower number of double bonds in a given lipid group is shown by red and blue color respectively, as well as thick lines and asterisks. The vertical dashed line shows the median number of double bonds in all long MLS predictors. (b) Enzyme DNA sequence variation. Distributions of the dN/dS values of the enzymes linked to the lipid predictors of long MLS (darker colors), and the enzymes linked to other lipids detected in our dataset (lighter colors), in brain and non-neural tissues (liver, muscle, and kidney) in the long-living species. Asterisks indicate p-value range *p < 0.05, **p < 0.01, ***p < 0.001.
