Figure 1 | Scientific Reports

Figure 1

From: Superresolution Imaging Identifies That Conventional Trafficking Pathways Are Not Essential for Endoplasmic Reticulum to Outer Mitochondrial Membrane Protein Transport

Figure 1

vMIA traffics to and clusters at the OMM in mouse embryonic fibroblasts. (a) Human (HFF and HeLa) and mouse (MEF) fibroblasts were transfected transiently (19 hours) to express vMIA-EYFP and then immunolabeled for the OMM marker Tom20. The images show an optical slice obtained by deconvolution of a confocal z-stack. (b) A single optical plane from deconvolved z-stack of gSTED images showing clustered distribution of vMIA-EYFP and Tom20 (immunostained) on the OMM of a HeLa cell transfected as above. Zoom shows the merged image marked by white box in the merged channel. (c) Normalized intensity profile of the red and green pixels marked by the dotted line in the zoomed region in panel b. (d) Single optical plane from deconvolved z-stack of gSTED images showing clustered distribution of vMIA-EYFP on the OMM in a WT MEF. Zoom shows the region marked by white box. (e) A single plane from an MSIM z-stack images showing clustered distribution of vMIA-EGFP on the OMM of a transfected MEF cell. Zoom of a single mitochondrion (boxed) shows vMIA clustering and line marks the region used to plot the intensity profile in the panel d. (f) Normalized intensity profile of the mitochondrion along the pixels marked by the solid line in the zoomed region. (g) Plot showing vMIA cluster size (FWHM) in WT MEFs. The line in the box and red, cross mark indicate the median (177.0) and mean (179.9 ± 3.7), respectively (n = 75).

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