Figure 3 | Scientific Reports

Figure 3

From: Superresolution Imaging Identifies That Conventional Trafficking Pathways Are Not Essential for Endoplasmic Reticulum to Outer Mitochondrial Membrane Protein Transport

Figure 3

Expression of vMIA causes redistribution of Mfn2 on the OMM. MEFs transfected with Mfn2-YFP (pseudocolored green) and Tom20-mCherry (red), (a) without or (b) with vMIA-CFP (blue) were analyzed by confocal microscopy. (a) In MEFs not expressing vMIA, Mfn2 shows punctate distribution along the mitochondria; boxes mark the zoomed regions shown below. (b) Expression of vMIA causes Mfn2 to distribute more uniformly along the mitochondria; boxes mark the zoomed regions below. (c) Plot showing quantification of Mfn2 colocalization with OMM marker Tom20 in MEFs expressing or not expressing vMIA-CFP. The line in the box represents the median value of cells not expressing (13%) or expressing vMIA (73.9%) (n > 16 cells). ***Represents p < 0.001.

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