Figure 1

Selective motor neuron death in co-cultures with SOD1^G93A expression after 6 days in vitro. Co-cultures of astrocytes and spinal neurons were grown for 3 days in vitro (3 DIV) or 6 days (6 DIV) after neuron seeding. SOD1^G93A expression in the neurons (AST^NTGNEU^G93A), in astrocytes (AST^G93ANEU^NTG), or in both cell types (AST^G93ANEU^G93A) leads to significant selective motor neuron death compared to fully non-transgenic co-culture (AST^NTGNEU^NTG) after 6 DIV. Figure shows the ratio of motor neurons to total spinal neurons expressed as a percentage of the respective AST^NTGNEU^NTG co-culture (presented as mean ± SEM, n = 4–6, *p < 0.05, **p < 0.01 after a Student’s t-test).