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Figure 1

From: Hydrolysis of Hyaluronic Acid in Lymphedematous Tissue Alleviates Fibrogenesis via TH1 Cell-Mediated Cytokine Expression

Figure 1

Analysis of the mouse model of lymphedema. Images of the right mouse leg at 3 days postsurgery. (A) Sham-operated mouse (without lymphatic damage) and mouse with lymphedema. (B,C) Cross-sectional mouse leg histology. Proximal slides were visualized via Masson’s trichrome staining. Scale bar = 5000 μm. Collagen is denoted by blue staining. Percentages of fibrotic areas (blue areas) were determined using Image J software. (D) Western blot analysis of the lymphedema markers VEGFR-3 and LYVE-1, alpha-smooth muscle actin, and GAPDH as a loading control. (E) Quantitative real-time PCR analysis of the expression of various hyaluronic acid synthases.

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