Figure 6
From: ALS-linked FUS exerts a gain of toxic function involving aberrant p38 MAPK activation
Active p38 MAPK in neurons within post-mortem motor cortex tissues from individuals diagnosed with ALS-FUS. Brain sections from paraffin-embedded tissue samples obtained from two control (C3 and C12) and two ALS-FUS (A2 and A3) cases were probed with antibodies against phosphorylated, catalytically active p38 (P-p38; red), phosphorylated neurofilament H (SMI31; green) as a marker of neuroaxonal integrity, and the nuclear stain DAPI (blue). Scale bar represents 10 μm. Low magnification images are shown in Supplemental Figure S3 . The P-p38 signal is higher, and SMI31 signal lower, in both ALS cases compared to controls; see Supplemental Figure S4 for quantification.
