Figure 1
From: CFTR is involved in the regulation of glucagon secretion in human and rodent alpha cells
CFTR is present in mouse and human alpha cells, but absent in delta cells. (A) Immunofluorescence of CFTR in sections of human pancreas. Immunostaining for CFTR (A .A), glucagon (A .B) and somatostatin (A .C), the overlay showing co-localization (A .D). Representative images from 3 donors and 20 islets from each donor that were investigated. (B) Confocal immunolocalization of CFTR (yellow) and glucagon (green) in fixed single pancreatic human alpha cell. (C) Mean intensity ratio (P1/P2) of CFTR antibody in the plasma membrane (P1) compartment compared to cytosolic compartment (P2) in human alpha cells (n = 30 cells from N = 3 donors). (D) As in B, but image showing a single mouse alpha cells. (E) As in C, but the estimate was made in mouse alpha cells (n = 7 cells from N = 2 mice). Plasma membrane region was 0.5 μm. White scale bar indicates 50 μm in A and 5 μm in B and D.
