Table 1 Translocation channel diameter d and selectivity r of SecYEG and SecYEG-YidC complexes.

From: YidC and SecYEG form a heterotetrameric protein translocation channel

 

ψr (mV)

r

\(\tilde{g}\) (pS/mM)

d (Å)

SecYEG + ribosomes

−7.2 ± 2.3

2 ± 0.5

1.6 ± 0.1

8.4 ± 0.5

SecYEG + RNCFtsQ

−6.7 ± 1.1

1.9 ± 0.2

1.7 ± 0.1

8.6 ± 0.5

SecYEG/YidC + ribosomes

−9.2

2.5 ± 0.4

0.9 ± 0.2

5.1 ± 0.6

SecYEG/YidC + RNCFtsQ

−15 ± 3.9

5.3 ± 3.15

1.75 ± 0.1

8.1 ± 0.5

  1. The reversal potential ψ r was obtained by plotting the single channel current as a function of membrane voltage (Fig. 3b). Anion to cation permeability ratios r (mean ± standard deviation) were calculated according to Eq. 1. \(\tilde{g}\) is the single-channel equivalent conductance, which is defined as the ratio of the single channel conductance g and the average ionic strength of solution in both the cis and trans compartments and d is the pore diameter calculated according to Eq. 2.
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