Figure 4
From: Rapid adaptation to microgravity in mammalian macrophage cells
Rapid adaptation to microgravity. (A) Raw fluorescent measurements up to 90 mins after zymosan injection. The oxidative burst reaction showed a sharp downward spike (see Fig. 4a) immediately after transition from 1 g to 0 g in all four independent experiments. (B) Magnified view of the oxidative burst decline and recovery after 1 g to 0 g transition. Symbols illustrate for each PMT the last measurement before signal decline (circle), local minimum before the onset of the adaptation process (triangles), and the time point at which the signal recovers its initial value (squares). Average amplitude of the downward spike and recovery time were 2095 ± 987 RLU and 42 ± 15 s, respectively. (C) Oxidative burst decline and recovery illustrated on the signals’ first time derivative. The thick red line depicts the average of first derivatives of the four independent PMT measurements, while the shaded area shows the standard deviation. All four independent experiments exhibited an immediate inhibitory effect of microgravity on oxidative burst reaction (negative derivative), followed by full adaptation after 42 s +/− 15 s (derivative back to its initial value). T1 = centrifuge stop command, T2 = full stop of the centrifuge 30 s after centrifuge stop command.
