Figure 6
From: Rapid adaptation to microgravity in mammalian macrophage cells
Comparison of the oxidative burst reaction rate across experimental conditions. (A) Synchronized and normalized signals. To account for differences in reaction initiation times and nominal signal intensity variations, the raw PMT measurements were synchronized in time, setting the time of zymosan injection as t = 0, and normalized by the peak signal intensity. For GRE1, PMT3 was a clear outlier and removed from the analysis. (B) Mean reaction rates over the first 90 min. The individual normalized signals were filtered to remove high frequency noise and reaction rates were calculated as the first time derivative. Lines and shaded areas represent the average and standard deviation of the four individual reaction rates for each experimental condition. (C) Magnification of the reaction rate minima. For rapid 0 g–1 g-transition (Run1, blue line), the reaction rate reaches a minimum 3 min after the return to 1 g (blue arrow), followed by an increase. For stepwise increase of gravitational force (Run2, red line), the minimum of the reaction rate was detected between 0.3 and 0.5 g (red arrow), followed by an increase.
