Figure 4

Co-immunofluorescence of RIIβ and the Golgi apparatus. Representative co-immunofluorescence of RIIβ (magenta) and the Golgi marker 58K protein (green) in a PRKACA^WT tumour (A,B) and in an PRKACA^{L206R mut} tumour (C). The overview shows accumulation of RIIβ in localized areas close to the nucleus and the overlay with the Golgi apparatus marker reveals co-localization (white, arrowheads). A higher magnification of the same tumour shows the close proximity of RIIβ and the Golgi apparatus (B), while the RIIβ staining is absent in the L206R mutated tumour (C). Scale bar 20 µm (A) and 10 µm (B,C).