Figure 2 | Scientific Reports

Figure 2

From: Lack of XPC leads to a shift between respiratory complexes I and II but sensitizes cells to mitochondrial stress

Figure 2

Oxygen consumption is not altered in intact XP-C fibroblasts. (A) O2 consumption was measured in MRC-5 (WT) and XP4PA (unXP-C) as described, using 4 × 104 cells/well in XF Assay Medium containing 10 mM glucose, with the subsequent additions of 1 µM oligomycin (ATP synthase inhibitor), 200 nM CCCP (uncoupler) and 1 µM rotenone plus AA (CI and CIII inhibitors). The trace represents the mean ± SD of three independent experiments and a schematic illustration of respiratory parameters. (B) Calculated respiratory parameters: Basal (base line OCR minus rotenone plus AA OCR), ATP (ATP-linked OCR – base line OCR minus oligomycin OCR), MRC (maximum respiratory capacity – CCCP OCR minus rotenone plus AA OCR), SRC (spare respiratory capacity – CCCP PCR minus base line OCR), PL (proton leak – oligomycin OCR minus rotenone plus AA OCR), NMR (non-mitochondrial respiration – rotenone plus AA OCR). The data represent mean ± SD of 3 independent experiments. **p < 0.01.

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