Figure 5 | Scientific Reports

Figure 5

From: Lack of XPC leads to a shift between respiratory complexes I and II but sensitizes cells to mitochondrial stress

Figure 5

Accumulation of DNA damage in mtDNA in XP-C cells. DNA damage was measured by XL-PCR amplification of a 16.3 kbp fragment of the mtDNA (A, left, and C) and a 10.4 kbp fragment of the HPRT gene, for the nuclear genome (A, right). Data is presented as ratio of bands intensity of long fragment over small fragment loading control (MT-ND1 and HPRT to mt and nDNA, respectively) relative to WT. The data represent mean ± SD in transformed and primary cells (n = 3). (B) MtDNA copy number and MT-ND4L frequency was assessed as the relative amplification of MT-ND1 (mtDNA gene) and HPRT (nDNA gene), and of MT-ND4L gene (frequently deleted) vs. MT-ND1 gene (rarely deleted), as described. WT and AS405 gene expression fold change are implicit and were set to 0.0 (zero) as control reference. The data represent mean ± SD in transformed (WT, unXP-C, corrXP-C and XP-A, n = 4) and primary (AS405 and XP17VI, n = 4 and n = 6, respectively) cells *p < 0.05, **p < 0.01 and ***p < 0.001.

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