Figure 5 | Scientific Reports

Figure 5

From: Creation of mutant mice with megabase-sized deletions containing custom-designed breakpoints by means of the CRISPR/Cas9 system

Figure 5

FISH analysis of 2-Mb and 5-Mb deletions. (A) Schematic representation of the positions of BAC probes. BACs located within the sgRNAs are shown with green lines and letters, and those outside in red lines and letters. (B,C) Representative nucleus images of DNA FISH analysis of fibroblasts obtained from #48 embryo of Fig. 1 (B, 2-Mb deletion) and #13 embryo of Fig. 2 (C, 5-Mb deletion). Probes that we used are indicated at the top of the images with probe colors. Enlarged images of boxed areas are placed on the right.

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