Figure 8
From: Identifying Novel Transcriptional and Epigenetic Features of Nuclear Lamina-associated Genes
NL genes of different subgroups are localized to the nuclear periphery. (a) Representative DNA FISH images of one A1 gene (Shcbp1), one I1 gene (Foxp2) and two I2 genes (Tbx5 and Itga8). FISH signals are shown in red and anti-Lamin B immunofluorescence signals were shown in green. Scale bars: 5 μm. (b) Cumulative frequency distributions of distances between eight NL genes and the NL. A1, I1 and I2 genes were plotted in pink, blue and orange, respectively. Pair-wise Kolmogorov-Smirnov (K-S) tests among six NL genes (Shcbp1, Myb, Dync2h1, Foxp2, Tbx5 and Itga8): P > 0.05 in all cases. Pair-wise K-S test between Sp4 or Cntnap5C and each of the other six NL genes: P < 0.05 in all cases. FISH data of three A1 genes (Myb, Sp4 and Dync2h1) and one I1 gene (Cntnap5C) were obtained from our previous study14.
