Figure 2 | Scientific Reports

Figure 2

From: CRISPR-Cas9 cleavage efficiency correlates strongly with target-sgRNA folding stability: from physical mechanism to off-target assessment

Figure 2

Pearson correlation between the relative cleavage efficiency and the fractional population of functional (active) structures, namely, R-loop structures that contain base pair (1, −1) and at least 7 base pairs in the hybrid helix, for (A) EMX1.1, (B) EMX1.2, (C) EMX1.3, (D) EMX1.6, (E) VEGFA.1, (F) Renilla, and (G) mRFP. The numbers in the brackets are the Pearson correlation coefficients. The red dots denote the data for the unmutated sgRNA sequences.

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