Figure 6
From: Steering surface topographies of electrospun fibers: understanding the mechanisms
Application of fibrous electrospun membranes to O2 sensing: (a) Stability of impregnated PtTFPP in PBS and cell culture medium during incubation at 37 °C. (b) Intensity map and phosphorescence lifetime imaging of stained membrane in PBS buffer at 21 kPa O2 and after deoxygenation obtained by confocal PLIM. (c) Representative phosphorescence decay curves at 0 and 21% O2. (d,e) average phosphorescence intensities and lifetimes measured at 0 and 21% O2, respectively and Stern-Volmer relationship. (f) Confocal PLIM of live HCT116 cells cultivated on membranes. Images of cells (stained with Calcein Green) and membrane (red) and in PLIM (membrane only) are shown. (g) 3-D reconstruction of cells growing in membrane. Different projections are shown. Scale bar is in µm.
